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Chromatin Immunoprecipitation (ChIP) enables the mapping of target proteins/histone modifications to specific loci in the genome. Antibodies and protein A beads, which have a higher affinity for some immunoglobulin isotypes, are used to immunoprecipitate chromatin/DNA complexes. The DNA region of interest is determined using quantitative PCR.

Here are six protocols to help you get the best out of your experiment.

ChIP protocols

• ChIP tips by Dr Andy Bannister (Gurdon institute)
• Troubleshooting tips to ensure successful ChIP
• ChIP using plant samples - Arabidopsis
• Chromatin preparation from tissues for ChIP
• Cross-linking chromatin immunoprecipitation (x-ChIP)
• Native chromatin immunoprecipitation

If you're working in a different application, we now have over 70 protocols, including guides for ELISA, FACS, IP and WB, and Abcam's Scientific support team are adding more all the time!

New ChIP primers - fast, easy, and consistent

We can now provide validated ChIP primers which you can use to:

• Test antibodies for use in ChIP
• Control for antibodies already validated in ChIP