Anti-Blood Group A Antigen antibody [HE-193] (ab2521)

Overview

  • Product name
    Anti-Blood Group A Antigen antibody [HE-193]
    See all Blood Group A Antigen primary antibodies
  • Description
    Mouse monoclonal [HE-193] to Blood Group A Antigen
  • Specificity
    This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssman antigen.
  • Tested applications
    Suitable for: IHC-P, Agglutinationmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Tissue Culture Supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • Clonality
    Monoclonal
  • Clone number
    HE-193
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Agglutination Use at an assay dependent dilution.

Target

  • Relevance
    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Cellular localization
    Cell Membrane
  • Database links

References for Anti-Blood Group A Antigen antibody [HE-193] (ab2521)

This product has been referenced in:
  • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »

See 1 Publication for this product

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I have received the following information:

ab2521: the IHC(P) application was tested on human colon carcinoma tissue. The paraffin-embedded sections were deparaffinized by xylene, hydrated by 100%, 90%,...

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Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 an...

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Thank you for your enquiry. The format of ab2521 is condensed supernatant with 15 mM sodium azide. No more supplements or buffers are added or necessary. We do not store this antibody in 1/20 volume 1M tris buffer solution, pH 8. I hope this info...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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