Anti-Blood Group AB antigen antibody [Z5H-2/Z2A] (ab24223)

Overview

  • Product name
    Anti-Blood Group AB antigen antibody [Z5H-2/Z2A]
  • Description
    Mouse monoclonal [Z5H-2/Z2A] to Blood Group AB antigen
  • Tested applications
    Suitable for: ELISA, WB, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Antigen A B of blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab24223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Flow Cyt
IP
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (PMID 19608744).
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Images

    • ab24223 staining Blood Group AB antigen in A549 cells by Immunocytochemistry/ Immunofluorescence.
      Cells were rinsed in PBS and fixed in 4% paraformaldehyde in PBS (15 minutes), rinsed, and blocked in 5% lamb serum in PBS/Tween 20 (15 minutes, room temperature). Cells were rinsed three times in PBS before application of primary antibodies. ab24223 diluted 1/400 in 5% lamb serum in PBS and applied to the fixed cells overnight (4 °C). Specimens were rinsed three times in PBS, and Alexa 568-conjugated goat anti-mouse IgG secondary antibody diluted 1/800 in PBS was applied. Counterstained with DAPI.

    References

    This product has been referenced in:
    • Higgins MA  et al. Differential recognition and hydrolysis of host carbohydrate antigens by Streptococcus pneumoniae family 98 glycoside hydrolases. J Biol Chem 284:26161-73 (2009). ICC/IF ; Human . Read more (PubMed: 19608744) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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