Anti-Blood Group B Antigen antibody [HEB-29] (ab2524)

Overview

  • Product nameAnti-Blood Group B Antigen antibody [HEB-29]
    See all Blood Group B Antigen primary antibodies
  • Description
    Mouse monoclonal [HEB-29] to Blood Group B Antigen
  • SpecificityThis antibody recognizes human blood group B antigens. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
  • Tested applicationsSuitable for: Agglutination, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: Tissue Culture Supernatant
  • PurityTissue culture supernatant
  • Purification notesConcentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • ClonalityMonoclonal
  • Clone numberHEB-29
  • Myelomaunknown
  • IsotypeIgM
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent dilution. The minimum titre is 1:128 with erythrocytes of blood group B.
IHC-P Use at an assay dependent dilution.

Target

  • RelevanceBlood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
  • Database links

References for Anti-Blood Group B Antigen antibody [HEB-29] (ab2524)

This product has been referenced in:
  • Vanak J  et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). Read more (PubMed: 2475797) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

ab2524 recognizes the blood group B antigen. This antigen is expressed on erythrocytes when the person has blood group B or AB.

I suggest to use erythrocytes from blood group B as positive control.
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Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 an...

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I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to con...

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The hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"