Anti-Blood Group B Antigen antibody [HEB-29] (ab2524)
Key features and details
- Mouse monoclonal [HEB-29] to Blood Group B Antigen
- Suitable for: IHC-P, Agglutination
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-Blood Group B Antigen antibody [HEB-29]
See all Blood Group B Antigen primary antibodies -
Description
Mouse monoclonal [HEB-29] to Blood Group B Antigen -
Host species
Mouse -
Specificity
This antibody recognizes human blood group B antigens. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood.
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Tested applications
Suitable for: IHC-P, Agglutinationmore details -
Species reactivity
Reacts with: Human -
Immunogen
Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined. -
Clonality
Monoclonal -
Clone number
HEB-29 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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Agglutination |
Use at an assay dependent concentration.
The minimum titre is 1:128 with erythrocytes of blood group B. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
Agglutination
Use at an assay dependent concentration. The minimum titre is 1:128 with erythrocytes of blood group B. |
Target
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Relevance
Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. -
Database links
- Entrez Gene: 28 Human
Datasheets and documents
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Datasheet download
References (7)
ab2524 has been referenced in 7 publications.
- Säljö K et al. Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands. Int J Mol Sci 22:N/A (2021). PubMed: 34208903
- Li C et al. Association between the ABO blood group and primary knee osteoarthritis: A case-control study. J Orthop Translat 21:129-135 (2020). PubMed: 32309138
- Jin C et al. Structural Diversity of Human Gastric Mucin Glycans. Mol Cell Proteomics 16:743-758 (2017). PubMed: 28461410
- Chen JT et al. Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response. Proc Natl Acad Sci U S A 112:13057-62 (2015). PubMed: 26438868
- Johansson MM et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J 32:393-412 (2015). PubMed: 26104834
- Barone A et al. Structural complexity of non-acid glycosphingolipids in human embryonic stem cells grown under feeder-free conditions. J Biol Chem 288:10035-50 (2013). PubMed: 23404501
- Vanak J et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88 (1989). PubMed: 2475797