Overview

  • Product name
    Anti-Blood Group H2 antigen antibody [BRIC231]
  • Description
    Mouse monoclonal [BRIC231] to Blood Group H2 antigen
  • Tested applications
    Suitable for: Agglutination, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) -HEL cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab33404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 6 family.
  • Domain
    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links
  • Alternative names
    • A transferase antibody
    • Abo antibody
    • B transferase antibody
    • BGAT_HUMAN antibody
    • CD173 antibody
    • Fucosylglycoprotein 3-alpha-galactosyltransferase antibody
    • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase antibody
    • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form antibody
    • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase antibody
    • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase antibody
    • Histo-blood group A transferase antibody
    • Histo-blood group B transferase antibody
    • NAGAT antibody
    see all

References

This product has been referenced in:
  • Gao C  et al. Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array. J Biol Chem 289:16462-77 (2014). Read more (PubMed: 24753245) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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