Anti-Blood Group Lewis a antibody [SPM522], prediluted (ab64099)

Overview

  • Product name
    Anti-Blood Group Lewis a antibody [SPM522], prediluted
    See all Blood Group Lewis a primary antibodies
  • Description
    Mouse monoclonal [SPM522] to Blood Group Lewis a, prediluted
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mucin isolated from an ovarian cyst.

  • Epitope
    Gal Beta1-3(Fuc alpha1-4) GlcNAc
  • Positive control
    • Human Colon carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Ready-to-use for 30 min at Room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
    • Cellular localization
      Cytoplasmic
    • Alternative names
      • Lewis A antigen antibody

    Images

    • ab64099 staining Blood Group Lewis a in human colon carcinoma tissue by immunohistochemistry on formalin-fixed, paraffin-embedded tissue.

    References

    This product has been referenced in:
    • Hamada E  et al. Investigation of unexpected serum CA19-9 elevation in Lewis-negative cancer patients. Ann Clin Biochem 49:266-72 (2012). Read more (PubMed: 22492877) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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