The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Ready-to-use for 30 min at Room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Lewis A antigen antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Blood Group Lewis a antibody [SPM522], prediluted (ab64099)
ab64099 staining Blood Group Lewis a in human colon carcinoma tissue by immunohistochemistry on formalin-fixed, paraffin-embedded tissue.
This product has been referenced in:
Hamada E et al. Investigation of unexpected serum CA19-9 elevation in Lewis-negative cancer patients. Ann Clin Biochem49:266-72 (2012).
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