Overview

  • Product nameAnti-Blood Group Lewis x antibody [P12]
  • Description
    Mouse monoclonal [P12] to Blood Group Lewis x
  • SpecificityThis antibody belongs to the BG-7 group and is specific for Lewis x (Type 2 Chain).
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fresh (non-cultured) placenta cell preparation (Human).

  • Positive control
    • Human kidney tissue.
  • General notes


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.0268% PBS, 1% BSA
  • PurityTissue culture supernatant
  • Primary antibody notesBlood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.
  • ClonalityMonoclonal
  • Clone numberP12
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40.
ICC/IF 1/200 - 1/400.

Target

  • Alternative names
    • Le(x) antibody
    • Lewis x Blood Group antibody

References for Anti-Blood Group Lewis x antibody [P12] (ab3358)

This product has been referenced in:
  • Shi CS  et al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood 112:3661-70 (2008). Read more (PubMed: 18711002) »
  • Nyström K  et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). Read more (PubMed: 17202152) »

See all 2 Publications for this product

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I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we ...

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I can confirm that ab3358Blood Group Lewis x antibody [P12] is sold as tissue culture supernatant. I have requested that the datasheetisupdated so thisinformation is clearer. Unpurified antibodies, such as those s...

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Thank you for your enquiry. This antibody is tissue culture supernatant concentrated by ammonium sulfate precipitation. The concentration of our last lot was 180 ug/ml. We don't currently have any stock of this antibody, so the concentration may ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"