Overview

  • Product nameAnti-Blood Group Lewis y antibody [F3]
  • Description
    Mouse monoclonal [F3] to Blood Group Lewis y
  • SpecificityThis antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SK-LU-3 lung cancer cell line (Human).

  • Positive control
    • Colon adenocarcinoma (human).
  • General notes


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • PurityPurified IgM
  • Primary antibody notesBlood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
  • ClonalityMonoclonal
  • Clone numberF3
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/40.
WB Use at an assay dependent concentration.
ICC/IF 1/200 - 1/400. PubMed: 17202152

Target

  • Alternative names
    • Lewis Y blood group antibody

References for Anti-Blood Group Lewis y antibody [F3] (ab3359)

This product has been referenced in:
  • Zhuang H  et al. The expression of annexin II and Lewis y antigen in ovarian epithelial tumors and the correlation between them. Tumour Biol 36:2343-9 (2015). Read more (PubMed: 25416440) »
  • Gao N  et al. c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer. Biochimie 107 Pt B:286-92 (2014). Read more (PubMed: 25239830) »

See all 11 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we ...

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Thank you for contacting us regarding the concentration of AB3359.

Although concentration of this product is lot dependent, our current lot ofab3359 Anti-Blood Group Lewis y antibody [F3] has a concentration of4.5ug/ml.

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Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"