Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Overview

  • Product nameAnti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

Properties

Applications

Our Abpromise guarantee covers the use of ab2179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP Use a concentration of 1 - 4 µg/ml.
ICC Use a concentration of 5 µg/ml.

Target

  • FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Anti-Blooms Syndrome Protein Blm antibody images



  • Predicted band size : 159 kDa
  • HeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).

  • HeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.

References for Anti-Blooms Syndrome Protein Blm antibody (ab2179)

This product has been referenced in:
  • Wu Y  et al. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair. PLoS Genet 11:e1005675 (2015). WB . Read more (PubMed: 26684013) »
  • Lu H  et al. Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice. Cell Death Dis 5:e1226 (2014). WB ; Human . Read more (PubMed: 24832598) »

See all 7 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (hek293)
Total protein in input 300 µg
Immuno-precipitation step Other - protein G dynabeads, 10 microliters
Specification hek293
Username

Dr. Edyta Marcon

Verified customer

Submitted Apr 30 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (8%)
Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
Specification Chinese Hamster Ovary cells (TRex CHO )
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (NTERA-2)
Loading amount 25 µg
Specification NTERA-2
Gel Running Conditions Reduced Denaturing (4-12%)
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (ntera2)
Loading amount 60000 cells
Specification ntera2
Treatment siRNA of Bloom
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Faisal Alzahrani

Verified customer

Submitted Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma cells)
Specification Colon carcinoma cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (8% gel)
Sample Human Cell lysate - whole cell (Colon carcinoma)
Specification Colon carcinoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Thank you for contacting Abcam regarding ab2179.

I am sorry that you have been experiencing difficulties with this antibody in ICC. I have reviewed the protocol information you provided and would like to make some suggestions to improve your...

Read More
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse testis)
Loading amount 100 µg
Specification mouse testis
Treatment Irradiation - 20 gy
Gel Running Conditions Reduced Denaturing (8% Precast gel (Invitrogen))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Application Immunocytochemistry
Sample Mouse Cell (mouse testis)
Specification mouse testis
Fixative Paraformaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HEK293-T)
Specification HEK293-T
Fixative Paraformaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 05 2011

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"