Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Overview

  • Product name
    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

Properties

Applications

Our Abpromise guarantee covers the use of ab2179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP Use a concentration of 1 - 4 µg/ml.
ICC Use a concentration of 5 µg/ml.

Target

  • Function
    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease
    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Images

  • All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179)

    Lane 1 : HeLa cells
    Lane 2 : 293T cells
    Lane 3 : Jurkat cells

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 159 kDa

    Detection:Chemiluminescence

    Exposure time:30 seconds

  • HeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).



  • Predicted band size : 159 kDa
  • HeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.

References

This product has been referenced in:
  • Gong Y  et al. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev 31:46-58 (2017). WB ; Mouse . Read more (PubMed: 28115467) »
  • Biehs R  et al. DNA Double-Strand Break Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination. Mol Cell 65:671-684.e5 (2017). Read more (PubMed: 28132842) »

See all 17 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (hek293)
Total protein in input
300 µg
Immuno-precipitation step
Other - protein G dynabeads, 10 microliters
Specification
hek293
Username

Dr. Edyta Marcon

Verified customer

Submitted Apr 30 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
Specification
Chinese Hamster Ovary cells (TRex CHO )
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (NTERA-2)
Loading amount
25 µg
Specification
NTERA-2
Gel Running Conditions
Reduced Denaturing (4-12%)
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (ntera2)
Loading amount
60000 cells
Specification
ntera2
Treatment
siRNA of Bloom
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Faisal Alzahrani

Verified customer

Submitted Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma cells)
Specification
Colon carcinoma cells
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Sample
Human Cell lysate - whole cell (Colon carcinoma)
Specification
Colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Thank you for contacting Abcam regarding ab2179.

I am sorry that you have been experiencing difficulties with this antibody in ICC. I have reviewed the protocol information you provided and would like to make some suggestions to improve your...

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse testis)
Loading amount
100 µg
Specification
mouse testis
Treatment
Irradiation - 20 gy
Gel Running Conditions
Reduced Denaturing (8% Precast gel (Invitrogen))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Application
Immunocytochemistry
Sample
Mouse Cell (mouse testis)
Specification
mouse testis
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (HEK293-T)
Specification
HEK293-T
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 05 2011

1-10 of 17 Abreviews or Q&A

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