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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Our Abpromise guarantee covers the use of ab2179 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
|ICC||Use a concentration of 5 µg/ml.|
HeLa cells cultured on coverslips were fixed in 4% paraformaldehyde then stained with ab2179 (green) at a concentration of 5µg/ml. The DNA stained with DAPI is shown in red (100x magnification).
HeLa cells were treated with 15µM Bleomycin for 2 hours to generate DNA double stranded breaks (DSBs). Paraformaldehyde-fixed cells were co-immunofluorescently labelled with anti-gamma H2AX (DNA DSB marker) and anti-BLM (ab2179). ab 2179 was used at a concentration of 5µg/ml. Colocalization at the sites of DNA DSBs is identified by the arrows. In the final panel DAPI is pseudo-coloured blue, while anti-gamma H2AX and anti-Blm are green and red respectively.