Anti-Blooms Syndrome Protein Blm antibody (ab476)

Overview

  • Product nameAnti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Tested applicationsSuitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Immunogen

    His-tagged 376 amino acid C-terminal of human blm fusion protein.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • FunctionParticipates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in diseaseDefects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Anti-Blooms Syndrome Protein Blm antibody images



  • Predicted band size : 159 kDa


    Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
  • BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.

References for Anti-Blooms Syndrome Protein Blm antibody (ab476)

This product has been referenced in:
  • Sturzenegger A  et al. DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end resection in human cells. J Biol Chem 289:27314-26 (2014). WB . Read more (PubMed: 25122754) »
  • Chaudhury I  et al. FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks. Mol Cell Biol 34:3939-54 (2014). Read more (PubMed: 25135477) »

See all 20 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 20 2013

Thank you for your call today and for letting us know about the trouble with the new lot of this antibody.

As we discussed, I'm sending a free of charge vial from a new lot of the antibody on order *** which should arrive early next week. Ple...

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Thank you for your reply.
Your credit note ID is ********.
I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the i...

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Thank you for your reply.
There will be a delay in you receiving these antibodies as ab2179 is currently out of stock, but it is due to come in on June 11th and we will hopefully send it to you the same day. I am sorry about the delay.
Your n...

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Thank you for calling Abcam earlier today.
I am sorry about the difficulty you have been having with a few of our antibodies and below are links to a few that would make a suitable alternative:
- http://www.abcam.com/Werner-s-syndrome-helicas...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Small Intestine)
Specification Small Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Oct 14 2010

As stated on the datasheet, "Cross reactivity with blm from other species has not been tested but is highly likely against mammalian homologues of human blm."

The blot shown on the datasheet is using HeLa Nuclear extracts, not whole cell extracts. However, there are several things to bear in mind: 1- It may be possible to dilute the antibody a bit more: We have used it at 1:2500 with good results. ...

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