Anti-Blooms Syndrome Protein Blm antibody (ab476)

Overview

  • Product name
    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Immunogen

    His-tagged 376 amino acid C-terminal of human blm fusion protein.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • Function
    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease
    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Images



  • Predicted band size : 159 kDa

    Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.

    Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
  • BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.

References

This product has been referenced in:
  • Hampp S  et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . Read more (PubMed: 27407148) »
  • Yang Y  et al. Arginine methylation facilitates the recruitment of TOP3B to chromatin to prevent R loop accumulation. Mol Cell 53:484-97 (2014). Read more (PubMed: 24507716) »

See all 28 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 20 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Oct 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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