Anti-Blooms Syndrome Protein Blm antibody (ab86664)

Overview

  • Product name
    Anti-Blooms Syndrome Protein Blm antibody
    See all Blooms Syndrome Protein Blm primary antibodies
  • Description
    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide designed within aa1061- aa1090 of human Blooms Syndrome Protein (conjugated to KLH).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86664 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 159 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • Involvement in disease
    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • Sequence similarities
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blm antibody
    • BLM_HUMAN antibody
    • Bloom syndrome antibody
    • Bloom syndrome protein antibody
    • Bloom syndrome RecQ helicase like antibody
    • BS antibody
    • DNA helicase antibody
    • DNA helicase RecQ like type 2 antibody
    • MGC126616 antibody
    • MGC131618 antibody
    • MGC131620 antibody
    • RECQ 2 antibody
    • RECQ like antibody
    • RecQ like type 2 antibody
    • RecQ protein like 3 antibody
    • RecQ protein-like 3 antibody
    • RecQ-like type 2 antibody
    • RecQ2 antibody
    • RECQL 2 antibody
    • RECQL 3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • type 2 antibody
    see all

Images

  • Anti-Blooms Syndrome Protein Blm antibody (ab86664) at 1/500 dilution + 35µg of HL60 whole cell lysate

    Secondary
    Goat anti-rabbit IgG conjugated to HRP

    Predicted band size : 159 kDa
    Additional bands at : 120 kDa,250 kDa. We are unsure as to the identity of these extra bands.
  • Flow cytometric analysis of Hela cells using ab86664 (1/50).

References

ab86664 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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