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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab86664 is purified through a protein A column, followed by peptide affinity purification.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 159 kDa.
1/10 - 1/50.
ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Function Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities Belongs to the helicase family. RecQ subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HRDC domain.
Post-translational modifications Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization Nucleus.
Information by UniProt
Bloom syndrome antibody
Anti-Blooms Syndrome Protein Blm antibody images
Western blot - Blooms Syndrome Protein Blm antibody (ab86664)
Anti-Blooms Syndrome Protein Blm antibody (ab86664) at 1/500 dilution + 35µg of HL60 whole cell lysate Secondary Goat anti-rabbit IgG conjugated to HRP Predicted band size : 159 kDa Additional bands at : 120 kDa,250 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry - Blooms Syndrome Protein Blm antibody (ab86664)
Flow cytometric analysis of Hela cells using ab86664 (1/50).
References for Anti-Blooms Syndrome Protein Blm antibody (ab86664)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"