ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.
Western blot - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
All lanes : Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Lane 1 : HepG2 cell lysate Lane 2 : HepG2 cell lysate with blocking peptide
Predicted band size: 159 kDa Observed band size: 159 kDa