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Rabbit polyclonal to BLZF1
Recombinant fragment corresponding to a region within amino acids 1-284 of Human BLZF1 (UniProt Q9H2G9).
K562, NCI-H929 and NIH-3T3 whole cell lysates; Hela cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
1/100 - 1/1000.
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Cytoplasm; Nucleus and Golgi apparatus lumen.
Information by UniProt
basic leucine zipper nuclear factor 1 (JEM 1) antibody
Basic leucine zipper nuclear factor 1 antibody
Western blot - BLZF1 antibody (ab155510)
All lanes : Anti-BLZF1 antibody (ab155510) at 1/1000 dilution Lane 1 : K562 whole cell lysate Lane 2 : NCI-H929 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 45 kDa
Western blot - Anti-BLZF1 antibody (ab155510)
Anti-BLZF1 antibody (ab155510) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size : 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BLZF1 antibody (ab155510)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling BLZF1 with ab155510 at 1/500 dilution (green). Right panel co-stained with Hoechst 33343 (blue).
has not yet been referenced specifically in any publications.
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