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Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with Human Bmf peptide (ab9656).|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 14668207Note: this was tested against sections embedded in polyester wax.|
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.