Anti-Bmi1 [1.T.21] antibody - ChIP Grade (ab14389)

Overview

  • Product nameAnti-Bmi1 [1.T.21] antibody - ChIP GradeSee all Bmi1 primary antibodies ...
  • Description
    Mouse monoclonal [1.T.21] to Bmi1 - ChIP Grade
  • SpecificityRecognizes mouse Bmi-1(triplet).
  • Tested applicationsICC/IF, Flow Cyt, ChIP, WB, ICC, IP, IHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cat, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-202 of Mouse Bmi1

  • Positive control
    • U2OS whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 0.65 µg/ml. PubMed: 19001505
Flow Cyt Use a concentration of 0.65 µg/ml. PubMed: 19001505
ChIP Use at an assay dependent dilution. PubMed: 18332116
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab14389 0.2ug/ml. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
ICC Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. PubMed: 17210912
IHC-P 1/100.

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Target information above from: UniProt accession P35226 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    see all

Anti-Bmi1 [1.T.21] antibody - ChIP Grade images

  • SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.55 Triton-X100 and incubated for 1 hour with ab14389 (1/200). The Bmi1 staining is shown in red. The cells were counterstained with DAPI (blue). 100x magnification.

  • ab14389 staining Bmi1 in human head and neck squamous cell cancers (HNSCC) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Sections were deparaffinized in xylene, dehydrated through graded alcohols, and placed in 0.1% hydrogen peroxide to quench any endogenous peroxidase activity. A 5 minute, 750 W microwave pretreatment in citrate buffer (pH 6.0) was repeated 4 times and followed by treatment with 10% normal rabbit serum for 30 minutes to block nonspecific antibody binding. The slides were then incubated with ab14389 at a 1/100. Primary antibody incubation was followed by three washes with PBST and incubation with fluorescently-labeled Cy2 (1/250) secondary antibody for 2 hours. Nuclei were counterstained with DAPI. Finally, sections were rinsed with PBST, dehydrated through sequential washes in 50%, 70%, 95%, and 100% ethanol and then cleared in xylene. Slides were mounted with DPX mounting media and allowed to dry overnight.
  • Anti-Bmi1 [1.T.21] antibody - ChIP Grade (ab14389) at 1/500 dilution + whole cell lysate prepared from Panc1 cancer cells at 35 µg

    Secondary
    HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 27 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Anti-Bmi1 [1.T.21] antibody - ChIP Grade (ab14389) at 1/500 dilution + whole tissue lysate prepared from murine pancreatic cancer at 50 µg

    Secondary
    HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Bmi1 [1.T.21] antibody - ChIP Grade (ab14389)

This product has been referenced in:
  • Hai B  et al. Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation. Clin Cancer Res 20:140-50 (2014). Read more (PubMed: 24150232) »
  • Li HY  et al. The Xenopus homologue of Down syndrome critical region protein 6 drives dorsoanterior gene expression and embryonic axis formation by antagonising polycomb group proteins. Development 140:4903-13 (2013). Xenopus laevis . Read more (PubMed: 24301465) »

See all 21 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (human acute promylocyitc leukemia NB4 cells)
Loading amount 40 µg
Specification human acute promylocyitc leukemia NB4 cells
Treatment 1 uM ATO treatment for indicated time
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. sungsin jo

Verified customer

Submitted Apr 02 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

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Application Western blot
Sample Mouse Tissue lysate - whole (pancreatic cancer sample)
Loading amount 50 µg
Specification pancreatic cancer sample
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2012

Application Western blot
Sample Human Cell lysate - whole cell (Panc1 cancer cell)
Loading amount 35 µg
Specification Panc1 cancer cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"