Anti-Bmi1 antibody (ab115251)
Key features and details
- Goat polyclonal to Bmi1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Bmi1 antibody
See all Bmi1 primary antibodies -
Description
Goat polyclonal to Bmi1 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
Immunogen
Synthetic peptide:
DSGSDKANSPAGG
, corresponding to amino acids 252-264 of Human Bmi1 -
Positive control
- Recombinant Human Bmi1 protein (ab114169) can be used as a positive control in WB. Human Skeletal Muscle tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 554342 Cat
- Entrez Gene: 493647 Chicken
- Entrez Gene: 510666 Cow
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 100173052 Orangutan
- Entrez Gene: 307151 Rat
see all -
Alternative names
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
Images
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Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ab115251 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115251 staining Bmi1 in Mouse Pancreatic intraepithelial neoplasia tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% BSA for 60 minutes at 26°C and antigen retrieval was by heat mediation in Tris-based buffer. The sample was incubated with primary antibody (1/200 in 10% BSA in TBST) at 4°C for 16 hours. A HRP-conjugated Donkey anti-goatpolyclonal (1/1000) was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab115251 has been referenced in 3 publications.
- Tanimura Y et al. Long-term model of colitis-associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells. Oncol Lett 21:7 (2021). PubMed: 33240413
- Yoshioka T et al. Bmi1 marks gastric stem cells located in the isthmus in mice. J Pathol 248:179-190 (2019). PubMed: 30689202
- Qiu M et al. MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells. Exp Ther Med 13:1329-1336 (2017). WB ; Human . PubMed: 28413473