The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1 - 1/1000.
RelevanceBone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.
Immunohistochemical analysis of BMP1 expression in Formalin-Fixed, Paraffin-Embedded Human Skin using 10 µg/ml ab118520 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemical analysis of BMP1 expression in Formalin-Fixed, Paraffin-Embedded Human Colon using 10 µg/ml ab118520 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-BMP1 antibody (ab118520)
This product has been referenced in:
Noda S et al. Transforming growth factor-ß signaling is upregulated in sporadic inclusion body myositis. Muscle NerveN/A:N/A (2016).
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Olderøy MØ et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One9:e91662 (2014).
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