Overview

  • Product nameAnti-BMP11 antibody
    See all BMP11 primary antibodies
  • Description
    Rabbit polyclonal to BMP11
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human BMP11 aa 32-61 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 10220

  • Positive control
    • WB: 293 cell lysate. IHC-P: human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionSecreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 11 antibody
    • BMP-11 antibody
    • BMP11 antibody
    • Bone morphogenetic protein 11 antibody
    • GDF 11 antibody
    • GDF-11 antibody
    • Gdf11 antibody
    • GDF11_HUMAN antibody
    • Growth differentiation factor 11 antibody
    • Growth/differentiation factor 11 antibody
    see all

Anti-BMP11 antibody images

  • All lanes : Anti-BMP11 antibody (ab71347) at 1/1000 dilution

    Lane 1 : 293 cell lysate, non transfected
    Lane 2 : 293 cell lysate, transiently transfected with the BMP11 gene

    Lysates/proteins at 2 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling BMP11 with ab71347. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling BMP11 with ab71347. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

References for Anti-BMP11 antibody (ab71347)

ab71347 has not yet been referenced specifically in any publications.

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I have gathered the following information:


ab124721: The immunogen range is located within the mature human protein (aa 299 - 407).


ab56645:immunogne is a recombinant fragment: ESRC...

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