Overview

  • Product nameAnti-BMP15 antibody [EPR5137]
    See all BMP15 primary antibodies
  • Description
    Rabbit monoclonal [EPR5137] to BMP15
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human BMP15.

  • Positive control
    • Ovary cancer, uterus, HL-60, NIH:OVCAR-3, Human testis, and HeLa cell lysates; Hela cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionMay be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.
    • Involvement in diseaseDefects in BMP15 are the cause of ovarian dysgenesis type 2 (ODG2) [MIM:300510]; also known as X-linked hypergonadotropic ovarian dysgenesis or hypergonadotropic ovarian failure due to ovarian dysgenesis. Ovarian dysgenesis leads to ovarian failure and accounts for about half of the cases of primary amenorrhea.
      Defects in BMP15 are the cause of premature ovarian failure type 4 (POF4) [MIM:300510]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BMP 15 antibody
      • BMP-15 antibody
      • BMP15 antibody
      • BMP15_HUMAN antibody
      • Bone morphogenetic protein 15 antibody
      • GDF 9B antibody
      • GDF-9B antibody
      • GDF9B antibody
      • Growth/differentiation factor 9B antibody
      • ODG2 antibody
      • POF4 antibody
      see all

    Anti-BMP15 antibody [EPR5137] images

    • All lanes : Anti-BMP15 antibody [EPR5137] (ab108413) at 1/1000 dilution

      Lane 1 : Ovary cancer lysate
      Lane 2 : Uterus lysate
      Lane 3 : HL-60 cell lysate
      Lane 4 : NIH:OVCAR-3 cell lysate
      Lane 5 : Human testis lysate
      Lane 6 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 45 kDa
    • Flow cytometric analysis of BMP15 in permeabilized Hela cells using ab108413 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-BMP15 antibody [EPR5137] (ab108413)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Gonad)
    Antigen retrieval step Enzymatic
    Permeabilization No
    Specification Gonad
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 14 2015

    Thank you for contacting us. The immunogen sequence has 100% homology with the sheep sequence, so I would expect reactivity. However, this cannot be guaranteed since we have not tested it in sheep.

    I hope this information is helpful to you. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"