Recombinant Anti-BMP15 antibody [EPR5137] (ab108413)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5137] to BMP15
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BMP15 antibody [EPR5137]
See all BMP15 primary antibodies -
Description
Rabbit monoclonal [EPR5137] to BMP15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Ovary cancer, uterus, HL-60, NIH:OVCAR-3, Human testis, and HeLa cell lysates; Hela cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5137 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. -
Involvement in disease
Defects in BMP15 are the cause of ovarian dysgenesis type 2 (ODG2) [MIM:300510]; also known as X-linked hypergonadotropic ovarian dysgenesis or hypergonadotropic ovarian failure due to ovarian dysgenesis. Ovarian dysgenesis leads to ovarian failure and accounts for about half of the cases of primary amenorrhea.
Defects in BMP15 are the cause of premature ovarian failure type 4 (POF4) [MIM:300510]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9210 Human
- Entrez Gene: 12155 Mouse
- Entrez Gene: 59302 Rat
- Omim: 300247 Human
- SwissProt: O95972 Human
- SwissProt: Q9Z0L4 Mouse
- Unigene: 532692 Human
- Unigene: 42160 Mouse
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Alternative names
- BMP 15 antibody
- BMP-15 antibody
- BMP15 antibody
see all
Images
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All lanes : Anti-BMP15 antibody [EPR5137] (ab108413) at 1/1000 dilution
Lane 1 : Ovary cancer tissue lysate
Lane 2 : Uterus tissue lysate
Lane 3 : HL-60 cell lysate
Lane 4 : NIH:OVCAR-3 cell lysate
Lane 5 : Human testis tissue lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
Intracellular flow cytometric analysis of BMP15 in permeabilized Hela cells using ab108413 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108413 has been referenced in 2 publications.
- Li D et al. MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma. Cancer Gene Ther 28:126-140 (2021). PubMed: 32616906
- Pelosi E et al. Constitutively active Foxo3 in oocytes preserves ovarian reserve in mice. Nat Commun 4:1843 (2013). Mouse . PubMed: 23673628