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Recombinant full length protein expressed in E. coli (Human).
Our Abpromise guarantee covers the use of ab6285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825|
|ICC/IF||Use a concentration of 1 µg/ml.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rabbit periosteum labeling BMP2 with ab6285 at 1/100 dilution. The tissue was fixed with paraformaldehyde, followed by blocking in 5% serum for 20 minutes. The tissue was incubated with ab6285 for 12 hours at 4°C in antibody dilution solution. An undiluted polyclonal goat-anti mouse HRP conjugate secondary antibody was used.