Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab14933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/2000.
ELISA 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa.Can be blocked with Mouse BMP2 peptide (ab80798).
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr 1/250 - 1/500.
IHC-P Use at an assay dependent concentration.

Target

Anti-BMP2 antibody images

  • ab14933 at 1/500 dilution staining human bone tissue sections by Immunohistochemistry (Frozen sections). The tissue was fixed with methanol and blocked with BSA prior to incubation with the antibody for 14 hours. StreptABComplex/HRP duet was used as the secondary antibody in this experiment. In the image specific staining is shown in brown at the site of new bone formation. The blue color is due to toluidine background staining.

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  • ICC/IF image of Rat primary hepatocytes culture cells stained with ab14933. The cells were fixed with 4% PFA.and incubated in  5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14933, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab14933 at 1/200 dilution staining BMP2 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/200 dilution.Cells were cultured in DMEM containing 10% FBS and Nuclei stained with DAPI.

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  • Anti-BMP2 antibody (ab14933) at 1/500 dilution + BMP2

References for Anti-BMP2 antibody (ab14933)

This product has been referenced in:
  • Zhang W  et al. Osteo-/odontogenic differentiation of BMP2 and VEGF gene-co-transfected human stem cells from apical papilla. Mol Med Rep 13:3747-54 (2016). WB ; Human . Read more (PubMed: 26986020) »
  • Lu H  et al. Hepcidin promotes osteogenic differentiation through the bone morphogenetic protein 2/small mothers against decapentaplegic and mitogen-activated protein kinase/P38 signaling pathways in mesenchymal stem cells. Mol Med Rep 11:143-50 (2015). WB ; Rat . Read more (PubMed: 25351366) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cartilage)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K 100ug/ml
Permeabilization No
Specification Cartilage
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative Formaldehyde
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Submitted Dec 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (aortic smooth muscle)
Gel Running Conditions Non-reduced Denaturing
Loading amount 20 µg
Specification aortic smooth muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted May 06 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Cell (MCF7 cells)
Specification MCF7 cells
Permeabilization Yes - 0.25% Triton, 15 min
Fixative Paraformaldehyde
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Submitted Jan 07 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF-7 cells)
Specification MCF-7 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 07 2015


For the detection of BMP2 in IHC I would recommend the Rabbit polyclonal ab14933 which is guaranteed to work in both IHC-Fr and IHC-P as well as on Mouse, Rat and Human samples. The link to the datasheet is www.abcam.com/ab14933.
I hope this ...

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Thank you for your enquiry and your interest.

We have not specifically tested this but as the immunogen for ab82511 (and ab14933) is designed to aa 45-60 and Swiss-Prot states the location of the amino acids that are glycosylated are at aa 13...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you very for contacting us.

This antibody is one of the high seller product and we haven't received many specificity complaint for this product so we are unsure why this particular vial is not working with your customer. I have few furth...

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Thank you for providing details quickly.

The protocol seems absolutely fine. The antibody should have worked, however we are not sure why this antibody is binding non specifically. In order to know the cause of multiple bands, is it possible ...

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Thank you for contacting us. I am sorry to hear that you are experiencing problems with this antibody.

I have checked the protocol and would like to ask few further questions for better understanding of the problem;

- "Reconsti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"