Overview

  • Product nameAnti-BMP2 antibody
    See all BMP2 primary antibodies
  • Description
    Rabbit polyclonal to BMP2
  • Specificityab82511 detects only the pro-form of the BMP2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Donkey, Goat, Deer, Badger
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BMP2.

    (Peptide available as ab90828.)

  • Positive control
    • This antibody gave a positive signal in Caco2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionInduces cartilage and bone formation.
  • Tissue specificityParticularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDA2 antibody
    • BMP-2 antibody
    • BMP-2A antibody
    • Bmp2 antibody
    • BMP2_HUMAN antibody
    • BMP2A antibody
    • Bone morphogenetic protein 2 antibody
    • Bone morphogenetic protein 2A antibody
    see all

Anti-BMP2 antibody images

  • IHC image of BMP2 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82511, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-BMP2 antibody (ab82511) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
    Bone morphogenetic protein 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Anti-BMP2 antibody (ab82511) at 1 µg/ml + Colon (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab82511 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82511, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BMP2 antibody (ab82511)

This product has been referenced in:
  • Kawakami Y  et al. A small interfering RNA targeting Lnk accelerates bone fracture healing with early neovascularization. Lab Invest 93:1036-53 (2013). Read more (PubMed: 23897412) »
  • Yang HY  et al. Proteomic analysis of gingival tissue and alveolar bone during alveolar bone healing. Mol Cell Proteomics 12:2674-88 (2013). IHC ; Pig . Read more (PubMed: 23824910) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest.

We have not specifically tested this but as the immunogen for ab82511 (and ab14933) is designed to aa 45-60 and Swiss-Prot states the location of the amino acids that are glycosylated are at aa 13...

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Merci de nous avoir contactés.

Je suis désolée de n'avoir pu améliorer vos résultats. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.
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Thank you for contacting us. Here is the link to the protein database UniProt entry for human BMP2 http://www.uniprot.org/uniprot/P12643 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"