Overview

  • Product nameAnti-BMP4 antibody [66119.11]
    See all BMP4 primary antibodies
  • Description
    Mouse monoclonal [66119.11] to BMP4
  • SpecificityMonoclonal Anti-BMP-4 neutralizes human BMP-4, and recognizes recombinant human BMP-4 by ELISA and immunoblotting. The antibody shows approximately 5% cross-reactivity with recombinant human BMP-2, recombinant mouse BMP RIA and BMP-RIB.
  • Tested applicationsSuitable for: WB, Neutralising, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein expressed in NSO cells (Human).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab6296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Use 3% BSA as blocking agent.
Neutralising Use a concentration of 1 - 3 µg/ml. to neutralise 50% of the bioactivity due to 0.5 µg/ml recombinant human BMP-4
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml. detects a limit of around 6 ng/well of human BMP-4.

Target

  • FunctionInduces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • Tissue specificityExpressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • Involvement in diseaseDefects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • zgc:100779 antibody
    • BMP 2B antibody
    • BMP 4 antibody
    • BMP-2B antibody
    • BMP-4 antibody
    • BMP2B antibody
    • BMP2B1 antibody
    • BMP4 antibody
    • BMP4_HUMAN antibody
    • Bone morphogenetic protein 2B antibody
    • Bone morphogenetic protein 4 antibody
    • DVR4 antibody
    • MCOPS6 antibody
    • MGC100779 antibody
    • OFC11 antibody
    • zbmp-4 antibody
    • ZYME antibody
    see all

References for Anti-BMP4 antibody [66119.11] (ab6296)

This product has been referenced in:
  • Bull TM  et al. Human Herpesvirus-8 Infection of Primary Pulmonary Microvascular Endothelial Cells. Am J Respir Cell Mol Biol : (2008). Read more (PubMed: 18587055) »

See 1 Publication for this product

Product Wall

Es freut mich wirklich zu höhren, dass ich Ihnen helfen konnte.
Ich wünsche viel Erfolg für die nachfolgenden Experimente!

1. Bleiben Sie bei einem Blockmittel während des Experimentes, also entweder Milch oder BSA, aber eben keine Kombination. Desweiteren scheinen monoklonale Antikörper bessere Ergebnisse mit 3% BSA zu liefern als mit Milch (siehe Anhang).

2. E...

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Das sollte eigentlich kein Problem darstellen: wir versenden unsere Produkte ja z. B. auch nach Australien oder Thailand, und da sind die auch schon ein mal bis zu 7 Tagen unterwegs, ohne das die Qualität leidet.

Wir haben dazu auch auch sch...

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Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer XXXXX und sollte ab dem 23. Mai bei Ihnen ankommen.

Al...

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The human cell line that your are using should be fine for this antibody. Please let me know the results of the no primary control and also if increasing the concentration of the primary works out.

Thank you for the details of your protocol. First, I suggest using a positive control. What species are your samples from? Ab6296 was tested on human recombinant purified BMP-4 (50 ng)at a concentration of 1-2 ug/ml. Second, is your secondary ant...

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Unfortunately, we do not have information regarding the binding site on BMP-4 for ab6296. I contacted the originators of this antibody but they were unable to help. We will update the on-line datasheet of this product as soon as we get more data. We a...

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As far as we are aware, ab6296 has not been tested in IHC. Please note that we would not recommned using a mouse monoclonal antibody in IHC on mouse tissue. This is becasuse the anti-mouse secondary would cross react with the endogenous mouse IgGs in t...

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This antibody has been used successfully in indirect immunoblotting to detect recombinant human BMP-4 as follows: 1-2 µg/ml primary antibody, using human BMP-4 at 50 ng/lane under non-reducing and reducing conditions. However, it has not been specifica...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"