Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab39973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 24376809
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • Tissue specificity
    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • Involvement in disease
    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • zgc:100779 antibody
    • BMP 2B antibody
    • BMP 4 antibody
    • BMP-2B antibody
    • BMP-4 antibody
    • BMP2B antibody
    • BMP2B1 antibody
    • BMP4 antibody
    • BMP4_HUMAN antibody
    • Bone morphogenetic protein 2B antibody
    • Bone morphogenetic protein 4 antibody
    • DVR4 antibody
    • MCOPS6 antibody
    • MGC100779 antibody
    • OFC11 antibody
    • zbmp-4 antibody
    • ZYME antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat bladder tissue sections labeling BMP4  with ab39973 at 1/100 dilution. The tissue was fixed in 10% buffered formalin overnight, processed and embedded in paraffin. Serial 6 µm sagittal tissue sections were sliced along the cranial-caudal axis of the bladder. (u) urothelium; (lp) lamina propria.

  • All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab39973 staining primary hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human brain tissue lysate - total protein (ab29466) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 8 : Human liver tissue lysate - total protein (ab29889) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 9 : Human heart tissue lysate - total protein (ab29431) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 10 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human BMP4 peptide (ab40140) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa



    ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution

    All lanes : mouse brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Dako Cytomation P0260
    Conjugation: HRP at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa


    Exposure time: 1 minute

References

This product has been referenced in:
  • Uemura MT  et al. Pericyte-derived bone morphogenetic protein 4 underlies white matter damage after chronic hypoperfusion. Brain Pathol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28470822) »
  • Zhang X  et al. Impact of siRNA targeting of ß-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Mol Med Rep 14:3595-601 (2016). WB ; Rat . Read more (PubMed: 27573468) »

See all 19 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 28 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (NF stage embryos)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - PBT (0.1% Triton in PBS)
Specification
NF stage embryos
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 07 2015

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 29 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (EPC2_hTERT immortalized esophageal keratinocytes)
Loading amount
20 µg
Specification
EPC2_hTERT immortalized esophageal keratinocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Kirill Pavlov

Verified customer

Submitted Jun 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal culture)
Specification
Hippocampal culture
Fixative
Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain (coronal section))
Specification
brain (coronal section)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jul 15 2008

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