Overview

  • Product nameBMP4 Human ELISA Kit
    See all BMP4 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    < 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.25 85% - 104%
    Serum 92.95 80% - 101%
    Plasma 94.32 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s BMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceBMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
  • Alternative names
    • BMP 2B
    • BMP 4
    • BMP2B
    • BMP2B1
    • BOMPR4A
    • Bone morphogenetic protein 2B
    • Bone morphogenetic protein 4
    • DVR4
    • OTTMUSP00000021065
    • OTTMUSP00000021066
    • ZYME
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

BMP4 Human ELISA Kit images

  • Representative Standard Curve using ab99982.

  • Representative Standard Curve using ab99982.

Protocols

References for BMP4 Human ELISA Kit (ab99982)

ab99982 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Both kits ab99982 and ab99983 use the same antibody pairs. The homology between mouse and human BMP4 is 98% and it is therefore likely that these kits will detect mouse BMP4 as well. This has not been tested in house...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"