Anti-BMP5 antibody (ab88064)

Overview

  • Product nameAnti-BMP5 antibody
    See all BMP5 primary antibodies
  • Description
    Mouse monoclonal to BMP5
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 323-454 of Human BMP5 with proprietary tag (NP_066551)

  • Positive control
    • Hela cells Nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab88064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 52 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-BMP5 antibody images

  • Anti-BMP5 antibody (ab88064) at 5 µg/ml + HeLa cells nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ICC/IF image of ab88064 staining of BMP5 in rat hippocampal culture. The sections were incubated in 0.3% triton X to permeabilise the cells and then in 10% serum to block non-specific protein-protein interactions. A two hour incubation at +24°C was performed with ab88064 (1:1000), followed by Alexa Fluor 568 conjugated secondary antibody. Red staining of the hippocampal neurons was observed.

    See Abreview

  • Detection limit for recombinant tagged BMP5 is approximately 1ng/ml as a capture antibody.

References for Anti-BMP5 antibody (ab88064)

ab88064 has not yet been referenced specifically in any publications.

Product Wall

I am sorry that you were having problems with ab88064, mouse BMP5. I have processed the free of charge replacement for ab93852, rabbit polyclonal to BMP5. Unfortunately, it now saying that it is  out of stock and there is a current lead time of 1-2 wee...

Read More

Thank you for contacting Abcam about ab88064, the anti-BMP5 antibody.   They have confirmed that the anti-BMP5 antibody has been tested in ELISA using recombinant BMP5 protein and they have provided the protocol that they used (see below). Please let m...

Read More

Thank you for contacting Abcam about ab88064.  Please find the protocol that was used the sandwich ELISA below. I have contacted the lab to see if the antibosy has been tested in a direct ELISA. If there is anything else I can do in the mean time pleas...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Hippocampal culture)
Specification Primary Hippocampal culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"