Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

Anti-BMP5 antibody images

  • All lanes : Anti-BMP5 antibody (ab93852) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human bone tumor tissue lysate - total protein (ab29359)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 50 kDa (possible cleavage fragment).

    Exposure time : 15 minutes
  • All lanes : Anti-BMP5 antibody (ab93852) at 1 µg/ml

    Lane 1 : Recombinant Human BMP5 protein (ab87627) at 0.1 µg
    Lane 2 : Recombinant Human BMP5 protein (ab87627) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-BMP5 antibody (ab93852)

ab93852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"