Human BMP5 ELISA Kit (ab119583)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMP5 ELISA Kit
See all BMP5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 242pg/ml 16.45 = 6.8% 2 16 1573pg/ml 69.21 = 4.4% 3 16 4363pg/ml 314.13 = 7.2% Inter-assay Sample n Mean SD CV% 1 24 266pg/ml 20.74 = 7.8% 2 24 1683pg/ml 82.46 = 4.9% 3 24 4290pg/ml 347.49 = 8.1% -
Sample type
Cell culture supernatant, Serum, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human BMP5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP5 in cell culture supernatants, serum and bone tissue.
A BMP5 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP5 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP5 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human BMP5 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human BMP5 antibody 1 x 100µl Lyophilized recombinant Human BMP5 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Induces cartilage and bone formation. -
Tissue specificity
Expressed in the lung and liver. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BMP 5
- BMP-5
- Bmp5
see all -
Database links
- Entrez Gene: 653 Human
- Omim: 112265 Human
- SwissProt: P22003 Human
- Unigene: 296648 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119583 has been referenced in 1 publication.
- Xie S et al. Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling. Int J Biol Sci 17:2367-2379 (2021). PubMed: 34239363