Overview

  • Product name
  • Description
    Rabbit polyclonal to BMP6
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human BMP6.

  • Positive control
    • This antibody gave a positive signal in Human spinal cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab101056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Induces cartilage and bone formation.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP-6 antibody
    • Bmp6 antibody
    • BMP6_HUMAN antibody
    • Bone morphogenetic protein 6 antibody
    • Bone Morphogenic Protein 6 antibody
    • Decapentaplegic vegetal related antibody
    • DVR6 antibody
    • HGNC:12686 antibody
    • TGFB related vegetal related growth factor antibody
    • Transforming growth factor beta antibody
    • Vegetal related (TGFB related) cytokine antibody
    • Vegetal related growth factor (TGFB related) antibody
    • Vg related sequence antibody
    • VG-1-R antibody
    • VG-1-related protein antibody
    • Vg1 related sequence antibody
    • VGR antibody
    • VGR-1 antibody
    • VGR1 antibody
    see all

Images

  • Anti-BMP6 antibody (ab101056) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab101056 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101056, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

References

ab101056 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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