Overview

  • Product nameBMP6 Human ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    200 pg/ml - 20000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.88 84% - 107%
    Serum 96.53 85% - 104%
    Plasma 94.69 84% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s BMP6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human BMP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceBone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.
  • Alternative names
    • BMP 6
    • Bone morphogenetic protein 6
    • Vegetal related (TGFB related) cytokine
    • Vegetal related growth factor (TGFB related)
    • VG 1 R
    • VG 1 related protein
    • Vg1 related sequence
    • VGR
    • VGR 1
    • VGR1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

BMP6 Human ELISA kit images

  • Representative standard curve using ab99984

Protocols

References for BMP6 Human ELISA kit (ab99984)

ab99984 has not yet been referenced specifically in any publications.

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