Anti-BMP7 antibody (ab54904)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
    • Tissue specificityExpressed in the kidney and bladder. Lower levels seen in the brain.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Developmental stageExpressed in the developing eye, brain and ear during embryogenesis.
    • Post-translational
      modifications
      Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BMP 7 antibody
      • BMP-7 antibody
      • Bmp7 antibody
      • BMP7_HUMAN antibody
      • Bone morphogenetic protein 7 antibody
      • Bone morphogenic protein 7 antibody
      • Eptotermin alfa antibody
      • OP 1 antibody
      • OP-1 antibody
      • OP1 antibody
      • Osteogenic protein 1 antibody
      see all

    Anti-BMP7 antibody images

    • IHC image of BMP7 staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54904, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



    • Predicted band size : 49 kDa
      BMP7 antibody (ab54904) at 1ug/lane + Raw cell lysate at 25ug/lane.
    • ICC/IF image of ab54904 staining of BMP7 in rat primary hippocampal neuron culture. The sections were incubated in 0.3% triton X to permeabilise the cells and in 10% serum to block non-specific protein-protein interactions. The sections were incubated with ab54904 (1:1000) overnight at +4°C, followed by a second incubation with Alexa 488 conjugated secondary antibody. BMP7 (green staining) is observed in the pyramidal cells of the hippocampus.

      See Abreview

    References for Anti-BMP7 antibody (ab54904)

    ab54904 has not yet been referenced specifically in any publications.

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    We haven’t tested this antibody for other BMP proteins.However, we have run blast with other BMP proteins.



    The similarity with BMP4 transcript variant 1/2/3 are all lower ...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Hippocampal neurons)
    Specification Hippocampal neurons
    Fixative Methanol
    Permeabilization Yes - 0.3% TritonX in 0.1% PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"