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Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.
Our Abpromise guarantee covers the use of ab56023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomA number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa