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Our Abpromise guarantee covers the use of ab56023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomA number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"