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Rabbit monoclonal [EPR13357] to BMP8b
This antibody detects dominant ~35kDa extra band in some samples, e.g. WI-38 and SK-OV-3.
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BMP8b aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P34820
HeLa and A549 cell lysate; A549 cells.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Belongs to the TGF-beta family.
Information by UniProt
Western blot - Anti-BMP8b [EPR13357] antibody (ab181253)
All lanes : Anti-BMP8b antibody [EPR13357] (ab181253) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : A549 cell lysate Lysates/proteins at 20 µg per lane. Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution Developed using the ECL technique Predicted band size : 45 kDa Observed band size : 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BMP8b [EPR13357] antibody (ab181253)
Immunofluorescence analysis of, paraformaldehyde-fixed, A549 cells labeling BMP8b with ab181253 at a 1/250 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200 dilution. In blue DAPI staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"