Overview

  • Product nameAnti-BMP9 antibody
    See all BMP9 primary antibodies
  • Description
    Rabbit polyclonal to BMP9
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    CKDDMGVPTLKYH

    , corresponding to amino acids 87-98 of Human BMP9 (corresponding to amino acids 406-417 of the full length propeptide)

Properties

Applications

Our Abpromise guarantee covers the use of ab35088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50 - 1/200.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20065063

Target

  • RelevanceBMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • BMP 9 antibody
    • Bone morphogenetic protein 9 antibody
    • GDF 2 antibody
    • GDF2 antibody
    • growth differentiation factor 2 antibody
    • Growth/differentiation factor 2 antibody
    see all

Anti-BMP9 antibody images

  • ab35088 (1/100) staining BMP9 in paraffin-embbeded human lung vascular lesion tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer. For further experimental details please refer to abreview.

    See Abreview

  • Immunofluorescent staining of BMP9 in rat primary hippocampus neurons with ab35088 (1/500). Cells were fixed with PFA and permeabilized with 0.3% Triton X in 0.1% PBS followed by a blocking step with 10% serum for 1 hour at 24oC.  Incubation with ab35088 (1/500) was then performed during 2 hours at 24oC. The secondary antibody was an anti rabbit-Alexa Fluro 488. DAPI shows nuclear localization.

    See Abreview

References for Anti-BMP9 antibody (ab35088)

This product has been referenced in:
  • Voorneveld PW  et al. The BMP pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in CRC. Br J Cancer 112:122-30 (2015). Read more (PubMed: 25393365) »
  • Mellott TJ  et al. IGF2 Ameliorates Amyloidosis, Increases Cholinergic Marker Expression and Raises BMP9 and Neurotrophin Levels in the Hippocampus of the APPswePS1dE9 Alzheimer's Disease Model Mice. PLoS One 9:e94287 (2014). WB ; Mouse . Read more (PubMed: 24732467) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Loading amount 20 µg
Specification Liver
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Apr 19 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Hippocampal culture)
Specification Primary Hippocampal culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung vascular lesion)
Specification lung vascular lesion
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - enzyme
Username

Dr. mark southwood

Verified customer

Submitted Aug 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"