Overview

  • Product nameAnti-BMPER antibody
    See all BMPER primary antibodies
  • Description
    Rabbit polyclonal to BMPER
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse BMPER.

    (Peptide available as ab74993.)

  • Positive control
    • This antibody gave a positive signal in the following Mouse Whole Cell Lysates: F9, NIH 3T3, EBS E14 TG2A Day 0

Properties

Applications

Our Abpromise guarantee covers the use of ab73900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75,110 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionInhibitor of bone morphogenetic protein (BMP) function, it may regulate BMP responsiveness of osteoblasts and chondrocytes.
  • Tissue specificityHighly expressed in lung, and brain and also in primary chondrocytes.
  • Involvement in diseaseDefects in BMPER are the cause of diaphanospondylodysostosis (DSD) [MIM:608022]. A rare, recessively inherited, perinatal lethal skeletal disorder. The primary skeletal characteristics of the phenotype include a small chest, abnormal vertebral segmentation, and posterior rib gaps containing incompletely differentiated mesenchymal tissue. Consistent craniofacial features include ocular hypertelorism, epicanthal folds, a depressed nasal bridge with a short nose, and low-set ears. The most commonly described extraskeletal finding is nephroblastomatosis with cystic kidneys, but other visceral findings have been described in some cases.
  • Sequence similaritiesContains 1 TIL (trypsin inhibitory-like) domain.
    Contains 5 VWFC domains.
    Contains 1 VWFD domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP binding endothelial regulator antibody
    • BMP binding endothelial regulator precursor protein antibody
    • BMP-binding endothelial regulator protein antibody
    • BMPER antibody
    • BMPER_HUMAN antibody
    • Bone morphogenetic protein binding endothelial cell precursor derived regulator antibody
    • Bone morphogenetic protein-binding endothelial cell precursor-derived regulator antibody
    • CRIM3 antibody
    • Crossveinless 2 antibody
    • CV 2 antibody
    • CV2 antibody
    • hCV2 antibody
    • Protein crossveinless 2 antibody
    • Protein crossveinless-2 antibody
    see all

Anti-BMPER antibody images

  • All lanes : Anti-BMPER antibody (ab73900) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 75,110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    BMPER has a predicted molecular weight of 76 kDa, however it is extensively glycosylated and has been seen to run in the 100 kDa region (other commercially available antibodies).
  • ICC/IF image of ab73900 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73900, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BMPER antibody (ab73900)

ab73900 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Submitted Dec 20 2010

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