The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Use at an assay dependent concentration. PubMed: 24173804
1/50 - 1/200.
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5.
Involvement in diseaseDefects in BMPR1B are the cause of acromesomelic chondrodysplasia with genital anomalies (AMDGA) [MIM:609441]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). Defects in BMPR1B are a cause of brachydactyly type A2 (BDA2) [MIM:112600]. Brachydactylies (BDs) are a group of inherited malformations characterized by shortening of the digits due to abnormal development of the phalanges and/or the metacarpals. They have been classified on an anatomic and genetic basis into five groups, A to E, including three subgroups (A1 to A3) that usually manifest as autosomal dominant traits. BDA2 was described first in a large Norwegian kindred. BDA2 is caused by mutations in BMPR1B gene and studies demonstrate that these mutations function as dominant negatives in vitro and in vivo.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 GS domain. Contains 1 protein kinase domain.
ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Western blot - BMPR1B antibody [MM0055-3E12] (ab78417)
All lanes : Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/ml
Lane 1 : Human bone tumor tissue lysate - total protein (ab29359) Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 30 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
References for Anti-BMPR1B antibody [MM0055-3E12] (ab78417)
This product has been referenced in:
Chapellier M et al. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response. Stem Cell Reports4:239-54 (2015).
Read more (PubMed: 25601208) »
Osório C et al. Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development. Development140:4751-62 (2013).
Read more (PubMed: 24173804) »