Overview

  • Product nameAnti-BMPR1B antibody [MM0055-3E12]
    See all BMPR1B primary antibodies
  • Description
    Mouse monoclonal [MM0055-3E12] to BMPR1B
  • Specificityab78417 detects BMPR1B. No cross reactivity was found to BMPR1A (ALK3).
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human BMPR1B extracellular domain

Properties

Applications

Our Abpromise guarantee covers the use of ab78417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24173804
IHC-P 1/50 - 1/200.

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5.
  • Involvement in diseaseDefects in BMPR1B are the cause of acromesomelic chondrodysplasia with genital anomalies (AMDGA) [MIM:609441]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers).
    Defects in BMPR1B are a cause of brachydactyly type A2 (BDA2) [MIM:112600]. Brachydactylies (BDs) are a group of inherited malformations characterized by shortening of the digits due to abnormal development of the phalanges and/or the metacarpals. They have been classified on an anatomic and genetic basis into five groups, A to E, including three subgroups (A1 to A3) that usually manifest as autosomal dominant traits. BDA2 was described first in a large Norwegian kindred. BDA2 is caused by mutations in BMPR1B gene and studies demonstrate that these mutations function as dominant negatives in vitro and in vivo.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin receptor like kinase 6 antibody
    • Acvrlk6 antibody
    • ALK 6 antibody
    • ALK6 antibody
    • alk6tr antibody
    • BMP type-1B receptor antibody
    • BMPR IB antibody
    • BMPR-1B antibody
    • Bmpr1b antibody
    • BMPRIB antibody
    • BMR1B_HUMAN antibody
    • Bone morphogenetic protein receptor type 1B antibody
    • Bone morphogenetic protein receptor type IB antibody
    • Bone morphogenetic protein receptor type-1B antibody
    • BR 1b antibody
    • BR1b antibody
    • CDw 293 antibody
    • CDw293 antibody
    • CDw293 antigen antibody
    • CFK 43a antibody
    • CFK43a antibody
    • Serine/threonine receptor kinase antibody
    • zALK 6 antibody
    • zALK6 antibody
    see all

Anti-BMPR1B antibody [MM0055-3E12] images

  • ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • All lanes : Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/ml

    Lane 1 : Human bone tumor tissue lysate - total protein (ab29359)
    Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 30 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-BMPR1B antibody [MM0055-3E12] (ab78417)

This product has been referenced in:
  • Chapellier M  et al. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response. Stem Cell Reports 4:239-54 (2015). IHC-P ; Human . Read more (PubMed: 25601208) »
  • Osório C  et al. Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development. Development 140:4751-62 (2013). WB, ICC/IF ; Mouse . Read more (PubMed: 24173804) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"