- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-BMPR2 antibody
See all BMPR2 primary antibodies - DescriptionRabbit polyclonal to BMPR2
- Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Immunogen
Recombinate fragment, corresponding to amino acids 295 - 552 of Human BMPR2 (BC052985).
- Positive control
- Raji cell lysate IF/ICC: HeLa cell line
Properties
- FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab106266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 115 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
- FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
- Tissue specificityHighly expressed in heart and liver.
- Involvement in diseaseDefects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1. - Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. - Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 659 Human
- Entrez Gene: 12168 Mouse
- Entrez Gene: 140590 Rat
- Omim: 600799 Human
- SwissProt: Q13873 Human
- SwissProt: O35607 Mouse
- Unigene: 471119 Human
- Unigene: 391654 Mouse
see all -
Alternative names
- BMP type II receptor antibody
- BMP type-2 receptor antibody
- BMPR 2 antibody
see all
Anti-BMPR2 antibody images
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Immunocytochemistry/ Immunofluorescence - Anti-BMPR2 antibody (ab106266)ICC/IF image of ab106266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - BMPR2 antibody (ab106266)Anti-BMPR2 antibody (ab106266) at 1/500 dilution + Raji cell lysate
Predicted band size : 115 kDa
References for Anti-BMPR2 antibody (ab106266)
This product has been referenced in:
- Gao X et al. Bone morphogenetic protein signaling protects against cerulein-induced pancreatic fibrosis. PLoS One 9:e89114 (2014). Mouse . Read more (PubMed: 24586530) »
- Zhu YT et al. Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells. J Cell Biol 206:799-811 (2014). Human . Read more (PubMed: 25202030) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"