Overview

  • Product nameAnti-BMPR2 antibody
    See all BMPR2 primary antibodies
  • Description
    Rabbit polyclonal to BMPR2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Chimpanzee, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human BMPR2.

  • Positive control
    • This antibody gave a positive signal in Human, Mouse and Rat Liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). Abcam recommends using milk as the blocking agent (3%)

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
  • Tissue specificityHighly expressed in heart and liver.
  • Involvement in diseaseDefects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
    Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP type II receptor antibody
    • BMP type-2 receptor antibody
    • BMPR 2 antibody
    • BMPR 3 antibody
    • BMPR II antibody
    • BMPR-2 antibody
    • BMPR-II antibody
    • Bmpr2 antibody
    • BMPR2_HUMAN antibody
    • BMPR3 antibody
    • BMPRII antibody
    • BMR 2 antibody
    • BMR2 antibody
    • Bone morphogenetic protein receptor type 2 antibody
    • Bone morphogenetic protein receptor type II antibody
    • Bone morphogenetic protein receptor type-2 antibody
    • Bone morphogenic protein receptor type II serine threonine kinase antibody
    • BRK 3 antibody
    • BRK3 antibody
    • PPH 1 antibody
    • PPH1 antibody
    • Serine threonine kinase type II activin receptor like kinase antibody
    • T ALK antibody
    • TALK antibody
    • Type II activin receptor like kinase antibody
    see all

Anti-BMPR2 antibody images

  • All lanes : Anti-BMPR2 antibody (ab115239) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
    Additional bands at : 37 kDa,45 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

References for Anti-BMPR2 antibody (ab115239)

ab115239 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Sample Rat Tissue lysate - whole (Rat liver)
Specification Rat liver
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"