Overview

  • Product name
  • Description
    Rabbit polyclonal to BMPR2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 667-921 of Human BMPR2 (NP_001195).

  • Positive control
    • H1299, Raji and NIH 3T3 whole cell lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab96826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 115 kDa.

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
  • Tissue specificity
    Highly expressed in heart and liver.
  • Involvement in disease
    Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
    Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP type II receptor antibody
    • BMP type-2 receptor antibody
    • BMPR 2 antibody
    • BMPR 3 antibody
    • BMPR II antibody
    • BMPR-2 antibody
    • BMPR-II antibody
    • Bmpr2 antibody
    • BMPR2_HUMAN antibody
    • BMPR3 antibody
    • BMPRII antibody
    • BMR 2 antibody
    • BMR2 antibody
    • Bone morphogenetic protein receptor type 2 antibody
    • Bone morphogenetic protein receptor type II antibody
    • Bone morphogenetic protein receptor type-2 antibody
    • Bone morphogenic protein receptor type II serine threonine kinase antibody
    • BRK 3 antibody
    • BRK3 antibody
    • PPH 1 antibody
    • PPH1 antibody
    • Serine threonine kinase type II activin receptor like kinase antibody
    • T ALK antibody
    • TALK antibody
    • Type II activin receptor like kinase antibody
    see all

Images

  • ICC/IF image of ab96826 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96826 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BMPR2 antibody (ab96826) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 115 kDa
    7.5% SDS-PAGE
  • Anti-BMPR2 antibody (ab96826) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 115 kDa
    7.5% SDS-PAGE

References

This product has been referenced in:
  • Awad KS  et al. Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol 310:L187-201 (2016). ICC/IF ; Human . Read more (PubMed: 26589479) »
  • Park ES  et al. Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling. Fertil Steril 100:1468-75 (2013). Read more (PubMed: 23993924) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - whole cell (neuron)
Gel Running Conditions
Reduced Denaturing (gradient 5-15%)
Loading amount
40 µg
Treatment
10nm to 10´M Arsenic
Specification
neuron
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
Yes - 0.3% Triton-X
Specification
skin
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Rachel1

Verified customer

Submitted Mar 24 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up