Overview

  • Product nameAnti-BMX antibody
    See all BMX primary antibodies
  • Description
    Rabbit polyclonal to BMX
  • Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the N-terminal region of human BMX

  • Positive control
    • WB: extracts from COS-7 cells; IHC-P: human thyroid gland tissue; ICC/IF: A549 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IP Use at an assay dependent concentration. PubMed: 21788516
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
IHC-P 1/50 - 1/1000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionActivity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
  • Tissue specificityPreferentially expressed in epithelial and endothelial cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • DomainSH2 domain mediates interaction with RUFY1.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bmx antibody
    • BMX non receptor tyrosine kinase antibody
    • BMX_HUMAN antibody
    • BONE MARROW KINASE, X-LINKED antibody
    • Bone marrow tyrosine kinase gene in chromosome X protein antibody
    • Cytoplasmic tyrosine protein kinase BMX antibody
    • Cytoplasmic tyrosine-protein kinase BMX antibody
    • Epithelial and endothelial tyrosine kinase antibody
    • ETK antibody
    • NKT38 antibody
    • NTK 38 antibody
    • NTK38 antibody
    • Protein tyrosine kinase BMX antibody
    • PSCTK 2 antibody
    • PSCTK 3 antibody
    • PSCTK2 antibody
    • PSCTK3 antibody
    see all

Anti-BMX antibody images

  • All lanes : Anti-BMX antibody (ab73887) at 1/500 dilution

    Lane 1 : extracts from COS7 cells
    Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Left panel: immunofluorescence analysis of A549 cells, using 1/550-1/1000 ab73887. Right panel: same, after pre-incubation with the immunizing peptide.
  • Left panel: immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using 1/50-1/100 ab73887; Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-BMX antibody (ab73887)

This product has been referenced in:
  • Hart JR  et al. Essential role of Stat3 in PI3K-induced oncogenic transformation. Proc Natl Acad Sci U S A 108:13247-52 (2011). WB, IP ; Mouse . Read more (PubMed: 21788516) »
  • Lage K  et al. Dissecting spatio-temporal protein networks driving human heart development and related disorders. Mol Syst Biol 6:381 (2010). IHC-P ; Human . Read more (PubMed: 20571530) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount 20 µg
Specification brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. Maryna Polyakova

Verified customer

Submitted Jun 26 2012

Thank you for contacting us to help to sovle this problem.

I am sorry that the customer is having troubles obtaining good results with this antibody. I have seen that you have already contacted us regarding this antibody earlier this year, ca...

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Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application o...

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Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer had difficulty obtaining satisfactory results fromanother antibody.

The details you have kindly provided will provide us with vital in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"