Recombinant Anti-BMX antibody [Y396] (ab32153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y396] to BMX
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BMX antibody [Y396]
See all BMX primary antibodies -
Description
Rabbit monoclonal [Y396] to BMX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human BMX aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y396 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa). |
Target
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Function
Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells. -
Tissue specificity
Preferentially expressed in epithelial and endothelial cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Domain
SH2 domain mediates interaction with RUFY1. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 660 Human
- Entrez Gene: 12169 Mouse
- Entrez Gene: 367786 Rat
- Omim: 300101 Human
- SwissProt: P51813 Human
- SwissProt: P97504 Mouse
- Unigene: 495731 Human
- Unigene: 504 Mouse
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Alternative names
- Bmx antibody
- BMX non receptor tyrosine kinase antibody
- BMX_HUMAN antibody
see all
Images
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Anti-BMX antibody [Y396] (ab32153) at 1/500 dilution + HeLa cell lysate
Predicted band size: 78 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab32153 has been referenced in 3 publications.
- Peng J et al. EPHA3 regulates the multidrug resistance of small cell lung cancer via the PI3K/BMX/STAT3 signaling pathway. Tumour Biol 37:11959-11971 (2016). PubMed: 27101199
- Xiong Y et al. Endotoxin Tolerance Inhibits Lyn and c-Src Phosphorylation and Association with Toll-Like Receptor 4 but Increases Expression and Activity of Protein Phosphatases. J Innate Immun 8:171-84 (2016). PubMed: 26457672
- Fiallos E et al. Human cytomegalovirus gene expression in long-term infected glioma stem cells. PLoS One 9:e116178 (2014). PubMed: 25549333