Overview

  • Product name
    Anti-BMX (phospho Y566) antibody
    See all BMX primary antibodies
  • Description
    Rabbit polyclonal to BMX (phospho Y566)
  • Specificity
    This antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).

  • Positive control
    • Human thyroid gland tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).

Target

  • Function
    Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
  • Tissue specificity
    Preferentially expressed in epithelial and endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Domain
    SH2 domain mediates interaction with RUFY1.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bmx antibody
    • BMX non receptor tyrosine kinase antibody
    • BMX_HUMAN antibody
    • BONE MARROW KINASE, X-LINKED antibody
    • Bone marrow tyrosine kinase gene in chromosome X protein antibody
    • Cytoplasmic tyrosine protein kinase BMX antibody
    • Cytoplasmic tyrosine-protein kinase BMX antibody
    • Epithelial and endothelial tyrosine kinase antibody
    • ETK antibody
    • NKT38 antibody
    • NTK 38 antibody
    • NTK38 antibody
    • Protein tyrosine kinase BMX antibody
    • PSCTK 2 antibody
    • PSCTK 3 antibody
    • PSCTK2 antibody
    • PSCTK3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.
  • Anti-BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 78 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-BMX (phospho Y566) antibody (ab59409)

    Lane 1 : Extracts from HeLa cells treated with Serum 20% 15'
    Lane 2 : Extracts from HeLa cells treated with Serum 20% 15' and with the synthesized peptide.


    Predicted band size : 78 kDa

References

This product has been referenced in:
  • Holopainen T  et al. Endothelial Bmx tyrosine kinase activity is essential for myocardial hypertrophy and remodeling. Proc Natl Acad Sci U S A 112:13063-8 (2015). Read more (PubMed: 26430242) »
  • Guryanova OA  et al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21481791) »

See all 2 Publications for this product

Customer reviews and Q&As

Bitte entschuldigen Sie die Verzögerung.

Ich habe nun aus dem Labor die Protokolle sowohl für die Behandlung der HeLa Zellen als auch das WB Protokoll für ab59409 bekommen.

Ich kopiere ihnen beide Protokolle unten in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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