BCL2/adenovirus E1B 19 kDa protein interacting protein 2 antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 antibody
BCL2/adenovirus E1B 19kDa interacting protein 2 antibody
BNIP 2 antibody
Anti-BNIP2 antibody images
Western blot - BNIP2 antibody (ab74820)
All lanes : Anti-BNIP2 antibody (ab74820) at 1/500 dilution
Lane 1 : extracts from Jurkat cells Lane 2 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 36 kDa Observed band size : 36 kDa Additional bands at : 46 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - BNIP2 antibody (ab74820)This image is courtesy of an anonymous Abreview
Central Panel: ab74820 staining BNIP2 in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS for 15 minutes and blocked with 2% BSA for 1 hour. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/250, was used as secondary antibody. Left-hand panel: Nuclei counterstained with DAPI (blue). Right-hand panel: Overlay
Immunocytochemistry - Anti-BNIP2 antibody (ab74820)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa
ab74820 staining BNIP2 (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (H&L) (1/750) was used as the secondary antibody.
WGA (red) = Wheat Germ Agglutinin