The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IP: 1/50 - 1/200.
WB: 1/1000 - 1/2000. Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Sequence similaritiesBelongs to the NIP3 family.
Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.