Overview

  • Product nameAnti-BNIP3 antibody - Carboxyterminal end
    See all BNIP3 primary antibodies
  • Description
    Rabbit polyclonal to BNIP3 - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    AIGLGIYIGRRLTTSTST

    corresponding to a sequence near the C-terminal of human BNIP3, identical to the related mouse and rat sequence.

  • Positive control
    • Mammary cancer tissue, rat thymus tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab65874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23691217

Target

  • FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
  • Sequence similaritiesBelongs to the NIP3 family.
  • Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
    • BNIP 3 antibody
    • BNIP3 antibody
    • BNIP3_HUMAN antibody
    • NIP 3 antibody
    • NIP3 antibody
    see all

Anti-BNIP3 antibody - Carboxyterminal end images

  • Anti-BNIP3 antibody - Carboxyterminal end (ab65874) at 2 µg/ml + rat thymus tissue lysate at 30 µg

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa (possible dimer).
  • ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BNIP3 antibody - Carboxyterminal end (ab65874)

This product has been referenced in:
  • Salem AF  et al. Cigarette smoke metabolically promotes cancer, via autophagy and premature aging in the host stromal microenvironment. Cell Cycle 12:818-25 (2013). Read more (PubMed: 23388463) »
  • Fedorova LV  et al. Peroxisome proliferator-activated receptor d agonist, HPP593, prevents renal necrosis under chronic ischemia. PLoS One 8:e64436 (2013). IHC-P ; Rat . Read more (PubMed: 23691217) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung)
Permeabilization Yes - .01% SDS
Specification Lung
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative Paraformaldehyde
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Submitted Aug 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"