The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa).
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration. PubMed: 23691217
FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Sequence similaritiesBelongs to the NIP3 family.
Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BNIP3 antibody - Carboxyterminal end (ab65874)
This product has been referenced in:
Salem AF et al. Cigarette smoke metabolically promotes cancer, via autophagy and premature aging in the host stromal microenvironment. Cell Cycle12:818-25 (2013).
Read more (PubMed: 23388463) »
Fedorova LV et al. Peroxisome proliferator-activated receptor d agonist, HPP593, prevents renal necrosis under chronic ischemia. PLoS One8:e64436 (2013).
Read more (PubMed: 23691217) »