Overview

  • Product nameAnti-BNIP3L antibody
    See all BNIP3L primary antibodies
  • Description
    Rabbit polyclonal to BNIP3L
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila C Virus
  • Immunogen

    peptide (

    DAQHESGQSSSRGSSH

    ) corresponding to amino acids 77 to 92 of human origin,which are identical to those of mouse Bnip3L (3).

  • Positive control
    • K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4).
  • General notes


    Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa.Can be blocked with BNIP3L peptide (ab8431). To reduce possible background load less sample and or use more diluted antibody.
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-P Use a concentration of 2 µg/ml.

Target

  • FunctionInduces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
  • Sequence similaritiesBelongs to the NIP3 family.
  • Post-translational
    modifications
    Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
  • Cellular localizationNucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenovirus E1B19k binding protein B5 antibody
    • Adenovirus E1B19K-binding protein B5 antibody
    • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
    • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
    • BNI3L_HUMAN antibody
    • BNIP3a antibody
    • BNIP3H antibody
    • BNIP3L antibody
    • BNIP3L protein antibody
    • NIP3 like protein X antibody
    • NIP3-like protein X antibody
    • NIP3L antibody
    • NIX antibody
    see all

Anti-BNIP3L antibody images

  • All lanes : Anti-BNIP3L antibody (ab8399) at 1 µg/ml

    Lane 1 : K562 whole cell lysate with absence of immunogenic peptide
    Lane 2 : K562 whole cell lysate with presence of immunogenic peptide
  • ab8399 at 2µg/ml staining BNIP3L in human kidney tissue by IHC 

  • ab8399 staining BNIP3L (NIX) in Mouse MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X100 and blocked with 10% Horse serum in 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500: in 10% Horse serum in 5% BSA ) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.

    See Abreview

  • Immunofluorescence of Bnip3L in Human Kidney cells using ab8399 at 10 ug/ml.

  • ab14404 staining BNIP3L in the H9C2 cell line derived from Rat Cardio tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 5% Serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in 5% Horse serum) for 2 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.

    See Abreview

References for Anti-BNIP3L antibody (ab8399)

This product has been referenced in:
  • Wu HM  et al. Hypoxia-induced autophagy mediates cisplatin resistance in lung cancer cells. Sci Rep 5:12291 (2015). WB ; Human . Read more (PubMed: 26201611) »
  • Chen Z  et al. EAF2 suppresses hypoxia-induced factor 1a transcriptional activity by disrupting its interaction with coactivator CBP/p300. Mol Cell Biol 34:1085-99 (2014). Read more (PubMed: 24421387) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hek293)
Specification hek293
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton-x100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Drosophila C Virus Tissue lysate - whole (fly heart)
Loading amount 10 µg
Specification fly heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (H9C2)
Loading amount 30 µg
Specification H9C2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hek293)
Loading amount 30 µg
Specification hek293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2013

Thank you for your reply and for confirming that information.

I've issued credit note # *** for this order. The full purchase price will be refunded back to your credit card pending approval of our accounting department. If you have any quest...

Read More

Thank you for contacting us and letting us know about the trouble with ab8399. I am sorry to see that the results were so disappointing.

I will be happy to issue a refund for your purchase. Please confirm your order information and I will pro...

Read More

Thank you for contacting Abcam.

Based on the information we have on this antibody, it is not uncommon for this antibody to recognize more than one band, but I would agree with your assessment that the band closet to 37kDa is the correct size ...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our products.

Reviewing this case, I would like to offer some suggestions to help optim...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Foreskin Fibroblasts)
Specification Foreskin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Dr. Sharon Sneddon

Verified customer

Submitted Nov 30 2011

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"