• Product nameAnti-BNIP3L antibody [EPR4033]
    See all BNIP3L primary antibodies
  • Description
    Rabbit monoclonal [EPR4033] to BNIP3L
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C-terminus in Human BNIP3L

  • Positive control
    • Raji and K562 cell lysates; Human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Associated products


Our Abpromise guarantee covers the use of ab109414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/100 - 1/250. antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionInduces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
    • Sequence similaritiesBelongs to the NIP3 family.
    • Post-translational
      Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
    • Cellular localizationNucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenovirus E1B19k binding protein B5 antibody
      • Adenovirus E1B19K-binding protein B5 antibody
      • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
      • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
      • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
      • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
      • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
      • BNI3L_HUMAN antibody
      • BNIP3a antibody
      • BNIP3H antibody
      • BNIP3L antibody
      • BNIP3L protein antibody
      • NIP3 like protein X antibody
      • NIP3-like protein X antibody
      • NIP3L antibody
      • NIX antibody
      see all

    Anti-BNIP3L antibody [EPR4033] images

    • All lanes : Anti-BNIP3L antibody [EPR4033] (ab109414) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 24 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414

    References for Anti-BNIP3L antibody [EPR4033] (ab109414)

    This product has been referenced in:
    • Pedanou VE  et al. The histone H3K9 demethylase KDM3A promotes anoikis by transcriptionally activating pro-apoptotic genes BNIP3 and BNIP3L. Elife 5:N/A (2016). Read more (PubMed: 27472901) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (4-20%)
    Sample Mouse Tissue lysate - whole (Lung)
    Specification Lung
    Treatment 10^7 CFU S. Aureus peritoneal clot
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Mr. Alan Chang

    Verified customer

    Submitted Mar 20 2014