The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
1/100 - 1/250. antigen retrieval is recommended.
Is unsuitable for Flow Cyt,ICC or IP.
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
Belongs to the NIP3 family.
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
Prieto-Domínguez N et al. Melatonin enhances sorafenib actions in human hepatocarcinoma cells by inhibiting mTORC1/p70S6K/HIF-1a and hypoxia-mediated mitophagy. Oncotarget8:91402-91414 (2017).
Read more (PubMed: 29207653) »
Pedanou VE et al. The histone H3K9 demethylase KDM3A promotes anoikis by transcriptionally activating pro-apoptotic genes BNIP3 and BNIP3L. Elife5:N/A (2016).
Read more (PubMed: 27472901) »