Overview

  • Product nameAnti-BNP antibody [24C5] (HRP)
    See all BNP primary antibodies
  • Description
    Mouse monoclonal [24C5] to BNP (HRP)
  • ConjugationHRP
  • SpecificityMAb 1B6 recognizes proBNP in Western Blotting after tricine-SDS-gel electrophoresis in reducing conditions.
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    FGRKMDRISSSS

    , corresponding to N terminal amino acids 11-22 of Human BNP

  • General notesImmunoglobulin concentrations vary from batch to batch (generally 5-8 mg/ml for this clone). Please contact technical services for the latest information.

Properties

Applications

Our Abpromise guarantee covers the use of ab14699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (ab20984).

    WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    References for Anti-BNP antibody [24C5] (HRP) (ab14699)

    This product has been referenced in:
    • Ding YD  et al. A sandwich ELISA for assessment of pharmacokinetics of HSA-(BNP)2 fusion protein in mouse plasma. J Pharm Biomed Anal 51:658-63 (2010). ELISA . Read more (PubMed: 19836183) »

    See 1 Publication for this product

    Product Wall

    Thank you for your email. For ab13111, the concentration of the batch that is currently in stock is 4.7 mg/ml. For ab14699, the concentration of the batch that is currently in stock is 5.80 mg/ml. If you have any additional questions, please contac...

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    Thank you for your enquiry. Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware,...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"